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Location:Home > People > Professor

Professor
Details
Name  
WANG Hailin
Title  
  Leader of DNA damage and molecular toxicology group
Highest Education  
  Ph.D
Office  
  18 Shuangqing Road, Haidian District, Beijing, China
Phone  
  0086-10-62849600
Zip Code  
  100085
Fax  
  0086-10-62849600
Email  
  HLWang@rcees.ac.cn

Education and Appointments:

Dr. Hailin Wang is a Professor of bioanalytical and environmental chemistry at the state key laboratory of environmental chemistry and ecotoxicology, Research Center for Eco-Environmental Sciences, CAS (2005-present). He obtained his Ph.D. from the Institute of Chemicals and Physics, Chinese Academy of Sciences (CAS) in 1997. He performed his postdoctoral research in the Department of Public Health Sciences, University of Alberta (Edmonton, Canada) in 2000 - 2004, and then served as a Research Associate in the University of Alberta (2004 - 2005). He has been awarded “Presidential Special Award for Graduate Student” by the CAS in 1997, “Hundreds of Talents” program by the CAS in 2005, and “Outstanding young scientist” by the Natural Science Foundation of China in 2011. He has published more than 75 papers in peer-reviewed leading journals, including Proc. Natl. Acad. Sci. USA, J. Am. Chem. Soc., Anal. Chem., Chem. Comm., J. Chromatogr. A, Electrophoresis. 


Research Interest:
Highly Sensitive DNA Modification Analysis, Single Molecule Detection; Protein-DNA interactions; DNA Repair Mechanistic Study. 
Public Services:

Honors:

1997, “Presidential Special Award for Graduate Student” by the CAS

2005, “Hundreds of Talents” program by the CAS

2011, “Outstanding young scientist” by the Natural Science Foundation of China

Selected Publications:

  1. Zhang, G.#; Huang, H.#; Liu, D.#; Cheng, Y.; Liu, X.; Zhang, W.; Yin, R.; Zhang, D.; Zhang, P.; Liu, J.; Li,C.; Liu, B.; Luo, Y.; Zhu, Y.; Zhang, N.; He, S.; He, C.; Wang, H.*; Chen, D.* N6-methyladenine DNA modification in Drosophila. Cell (2015), DOI: 10.1016/j.cell.2015.04.018. (# Co-first author; *Co-corresponding author) 

  2. Yin, R.; Mo, J.; Lu, M.; Wang, H.* Detection of human urinary 5-hydroxymethylcytosine by stable isotope dilution HPLC-MS/MS Analysis. Anal Chem. 87, 1846-1852 (2015). 

  3. Zhao, C.; Wang, H.*; Zhao, B. L.; Li, C. P.; Yin, R.; Song, M.; Liu, Z.; Jiang, G. B. Boronic acid-mediated polymerase chain reaction for gene- and fragment-specific detection of 5-hydroxymethylcytosine. Nucleic Acids Res., 42: e81 (2014). 

  4. Zhao, B. L.#; Yang, Y. #; Wang, X.#; Chong Z.; Yin, R.; Song, S. H.; Zhao, C.; Li, C.; Huang, H.; Sun, B. F. Wu, D.; Jin, K. X. Song, M.; Zhu, B. Z.; Jiang, G.; Danielsen, J. M. R.; Xu, G. L.; Yang, Y. G.*; Wang, H.* Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism. Nucleic Acids Res., 42:1593-1605 (2014). 

  5. Zou, D. D.; Zhang, D. P.; Liu, S. Q.; Zhao, B. L.; Wang, H.* Interplay of binding stoichiometry and recognition specificity for the interaction of MBD2b protein and methylated DNA revealed by affinity capillary electrophoresis coupled with laser-induced fluorescence analysis. Anal. Chem., 86:1775-1782 (2014). 

  6. Yin, R.; Mao, S. Q.; Zhao, B.; Chong, Z.; Yang, Y.; Zhao, C.; Zhang, D.; Huang, H.; Gao, J.; Li, Z,; Jiao, Y.; Li, C.; Liu, S.; Wu, D.; Gu, W.; Yang, Y. G.; Xu, G. L.; Wang, H.* Ascorbic acid enhances Tet-mediated 5-methylcytosine oxidation and promotes DNA demethylation in mammals. J. Am. Chem. Soc., 135:10396-10403 (2013). (Highlighted by Nature Reviews Genetics). 

  7. Liu, S.; Zhao, B.; Zhang, D.; Li, C.; Wang, H.* Imaging of non-uniform motion of single DNA molecules reveals the kinetics of varying-field isotachophoresis. J. Am. Chem. Soc., 135:4644-4647 (2013). 

  8. Yin, R.; Liu, S.; Zhao, C.; Lu, M.; Tang, M.-S.; Wang, H.* An ammonium bicarbonate enhanced stable isotope dilution UHPLC-MS/MS method for sensitive and accurate quantification of acrolein-DNA adducts in human leukocytes. Anal. Chem., 85:3190-3197 (2013). 

  9. Song, M.; Zeng, L.; Hong, X.; Meng, Z.; Yin, J.; Wang, H.*; Liang, Y.; Jiang, G. B. Polyvinyl pyrrolidone promotes DNA cleavage by a ROS-independent and depurination mechanism. Environ. Sci. Technol., 47: 2886-2891 (2013).  

  10. Zhang, D.; Zhao, Q.; Zhao, B.; Wang, H.* Fluorescence anisotropy reduction of allosteric aptamer for sensitive and specific protein signaling. Anal. Chem., 84:3070-3074 (2012). 

  11. Zhao, C.; Yin, R. C.; Yin, J. F.; Wang, H.* Capillary monolithic bioreactor of immobilizing snake phosphodiesterase for mass spectrometry based oligodeoxynucleotide sequencing. Anal. Chem., 84:1157-1164 (2012).  

  12. Zhang, D. P.; Lu, M. L.; Wang, H.* Fluorescence anisotropy analysis for mapping aptamer-protein interactions at the single nucleotide levels. J. Am. Chem. Soc., 133:9188-9191 (2011). 

  13. Wang, H., Lu, M., Tang, M. S., Van Houten, B., Ross, J. B. A., Weinfeld, M.; Le, X.C. DNA wrapping is required for DNA damage recognition in the E. coli DNA nucleotide excision repair pathway. Proc. Natl. Acad. Sci. USA, 106:12849-12854 (2009). 

  14. Wang, Z. X.; Lu, M.; Wang, X. L.; Yin, R. C.; Song, Y. L.; Le, X. C.; Wang, H.* Quantum dots enhanced ultrasensitive detection of DNA adducts. Anal. Chem., 81:10285-10289 (2009).  

  15. Wang, X. L.; Song, Y. L.; Song, M. Y.; Li, T.; Wang, Z. X.; Wang, H.* Fluorescent polarization combined capillary electrophoresis immunoassay for rapid and sensitive detection of genomic DNA methylation. Anal. Chem., 81:7885-7891 (2009). (Accelerated Article)  

  PEER-REVIEWED PUBLICATIONS 

  103. Zhang, G.#; Huang, H.#; Liu, D.#; Cheng, Y.; Liu, X.; Zhang, W.; Yin, R.; Zhang, D.; Zhang, P.; Liu, J.; Li,C.; Liu, B.; Luo, Y.; Zhu, Y.; Zhang, N.; He, S.; He, C.; Wang, H.*; Chen, D.* N6-methyladenine DNA modification in Drosophila. Cell (2015), http://dx.doi.org/10.1016/j.cell.2015.04.018. (# Co-first author; *Co-corresponding author) 

    

  102. Li, Z.; Gu, T. P.; Weber, A. R.; Shen, J. Z.; Li, B. Z.; Xie, Z. G.; Yin, R.; Guo, F.; Liu, X.; Tang, F.; Wang, H.; Schar, P.; Xu, G. L. Gadd45a promotes DNA demethylation through TDG. Nucleic Acids Res. DOI: 10.1093/nar/gkv283 

    

  101. Zhang, D.; Fu, R.; Zhao, Q.; Rong, H.; Wang, H.* Nanoparticles-free fluorescence anisotropy amplification assay for detection of RNA nucleotide-cleaving DNAzyme activity. Anal Chem. DOI: 10.1021/acs.analchem.5b00479 

    

  100. Yin, R.; Mo, J.; Lu, M.; Wang, H.* Detection of human urinary 5-hydroxymethylcytosine by stable isotope dilution HPLC-MS/MS Analysis. Anal Chem. 87, 1846-1852 (2015). 

    

  99. Liu, S.; Zhang, D.; Wang, H.* Transient isotachophoresis focusing of DNA and DNA-protein complexes is essentially enhanced by spontaneously dissolved aerial carbon dioxide in electrolytes. Anal Chem. 87, 554-560 (2015). 

    

  98. Li, J.; Wang, W.; Moe, B.; Wang, H.; Li, X. F. Chemical and toxicological characterization of halobenzoquinones, an emerging class of disinfection by products. Chem Res Toxicol, 28:306-318 (2015). 

    

  97. Chen J. Y.; Gao Y. W.; Huang H.; Jiang Y. H.; Li H.; Gao S.; Tao Y.; Wang H.; Wang H. L.; Cao T.; Gao S. R. The combination of Tet1 and Oct4 generates high-quality mouse induced pluripotent stem cells (iPSCs). Stem Cells, 33:686-698 (2015) 

    

  96. Zhao, Q.*; Lv, Q.; Wang, H. Aptamer fluorescence anisotropy sensors for adenosine triphosphate by comprehensive screening tetramethylrhodamine labeled nucleotides. Biosens Bioelectron, 70:188-193 (2015). 

    

  95. Lee, Y. H.; Wang, H. T.; Wang, M. W.; Hu, Y.; Chen, W. S.; Chou, D.; Liu, Y.; Donin, N.; Huang, W. C.; Lepor, H.; Wu, X. R.; Wang, H. L.; Beland, F. A.; Tang, M. S. Acrolein- and 4-aminobiphenyl-DNA adducts in human bladder mucosa and tumor tissue and their mutagenicity in human urothelial cells. Oncotarget, 2014, 5, 3526-3540 (2014). 

    

  94. Zou, D.; Wang, X.; Chen, Z.; Zhang, D.; Wang H.* Engineered SNAP-MBD2b proteins for specific recognition of methylated DNA. Sci. China Chem., 57:1019-1025 (2014).  

    

  93. Song, M.*, Liang D., Liang, Y., Chen, M., Wang, F., Wang, H., Jiang, G. Assessing developmental toxicity and estrogenic activity of halogenatedbisphenolA on zebrafish (Daniorerio). Chemosphere 112: 275-281(2014).  

    

  92. Zhao, C.; Wang, H.*; Zhao, B. L.; Li, C. P.; Yin, R.; Song, M.; Liu, Z.; Jiang, G. B. Boronic acid-mediated polymerase chain reaction for gene- and fragment-specific detection of 5-hydroxymethylcytosine. Nucleic Acids Res., 42: e81(2014). 

    

  91. Zou, D. D.; Zhang, D. P.; Liu, S. Q.; Zhao, B. L.; Wang, H.* Interplay of binding stoichiometry and recognition specificity for the interaction of MBD2b protein and methylated DNA revealed by affinity capillary electrophoresis coupled with laser-induced fluorescence analysis. Anal. Chem., 86:1775-1782 (2014). 

    

  90. Zhang, D. P.; Yin, L.; Meng, Z. H.; Yu, A. C.; Guo, L. H.; Wang, H.* A sensitive fluorescence anisotropy method for detection of lead (II) ion by a G-quadruplex-inducible DNA aptamer. Anal. Chim. Acta, 812:161-167 (2014). 

    

  89. Zhao, Q.*; Lv, Q.; Wang, H. Identification of allosteric nucleotide sites of tetramethylrhodamine-labeled aptamer for noncompetitive aptamer-based fluorescence anisotropy detection of a small molecule, ochratoxin A. Anal. Chem., 86:1238-1245 (2014). 

    

  88. Zhao, B. L.#; Yang, Y. #; Wang, X.#; Chong Z.; Yin, R.; Song, S. H.; Zhao, C.; Li, C.; Huang, H.; Sun, B. F. Wu, D.; Jin, K. X. Song, M.; Zhu, B. Z.; Jiang, G.; Danielsen, J. M. R.; Xu, G. L.; Yang, Y. G.*; Wang, H.* Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism. Nucleic Acids Res., 42:1593-1605 (2014). 

    

  87. Yin, R.; Mao, S. Q.; Zhao, B.; Chong, Z.; Yang, Y.; Zhao, C.; Zhang, D.; Huang, H.; Gao, J.; Li, Z,; Jiao, Y.; Li, C.; Liu, S.; Wu, D.; Gu, W.; Yang, Y. G.; Xu, G. L.; Wang, H.* Ascorbic acid enhances Tet-mediated 5-methylcytosine oxidation and promotes DNA demethylation in mammals. J. Am. Chem. Soc., 135:10396-10403 (2013). (Highlighted by Nature Reviews Genetics). 

    

  86. Liu, S.; Zhao, B.; Zhang, D.; Li, C.; Wang, H.* Imaging of non-uniform motion of single DNA molecules reveals the kinetics of varying-field isotachophoresis. J. Am. Chem. Soc., 135:4644-4647 (2013). 

    

  85. Gao, Y.; Chen, J.; Li, K.; Wu, T.; Huang, B.; Liu, W.; Kou, X.; Zhang, Y.; Huang, H.; Jiang, Y.; Yao, C.; Liu, X.; Lu, Z.; Kang, L.; Chen, J.; Wang, H.; Cao, T*.; Gao, S*. Replacement of Oct4 by Tet1 during iPSC induction reveals an important role of DNA methylation and hydroxymethylation in reprogramming. Cell Stem Cell, 12:453-469 (2013). 

    

  84. Yin, R.; Liu, S.; Zhao, C.; Lu, M.; Tang, M.-S.; Wang, H.* An ammonium bicarbonate enhanced stable isotope dilution UHPLC-MS/MS method for sensitive and accurate quantification of acrolein-DNA adducts in human leukocytes. Anal. Chem., 85:3190-3197 (2013). 

    

  83. Yin, J. F.; Wang, Z. X.; Song, M. Y.; Zhao, C., Wang, H.* Plastic antibody for DNA damage: fluorescent imaging of BPDE-dG adducts in genomic DNA. Analyst 138:4958-4966 (2013). 

    

  82. Song, M.; Zeng, L.; Hong, X.; Meng, Z.; Yin, J.; Wang, H.*; Liang, Y.; Jiang, G. B. Polyvinyl pyrrolidone promotes DNA cleavage by a ROS-independent and depurination mechanism. Environ. Sci. Technol., 47: 2886-2891 (2013).  

    

  81. Song, M.; Zeng, L.; Yuan, S.; Yin, J.; Wang, H.*; Jiang, G. B. Study of single-wall carbon nanotubes functionized with different chemical groups on human MCF7 cells. Chemosphere, 92: 576-582 (2013). 

    

  80. Li, T. P.; Wang, Z.; Zhao, Y.; He, W.; An, L.; Liu, S.; Liu, Y.; Wang, H.; Hang, H*. Checkpoint protein Rad9 plays an important role in nucleotide excision repair. DNA Repair (Amst) 12:284-292 (2013). 

    

  79. Wang, X.; Wang, H.* Epigenotoxicity of environmental pollutants evaluated by a combination of DNA methylation inhibition and capillary electrophoresis¨Claser-induced fluorescence immunoassay. Anal. Bioanal. Chem., 405:2435-2442 (2013). 

    

  78. Zhao, Q.*; Geng, X.; Wang, H. Fluorescent sensing ochratoxin A with single fluorophore-labeled aptamer. Anal. Bioanal. Chem. 405:6281-6286 (2013). 

    

  77. Geng, X.; Zhang, D.; Wang, H.; Zhao, Q.* Screening interaction between ochratoxin A and aptamers by fluorescence anisotropy approach. Anal. Bioanal. Chem., 405:2443-2449 (2013). 

    

  76. Yin, R.#; Zhang, D. #; Song, Y.; Zhu, B. Z.*; Wang, H. * Potent DNA damage by polyhalogenated quinones and H2O2 via a metal-independent and Intercalation-enhanced oxidation mechanism. Sci. Reports, 3:1269 (2013).  

    

  75. Agbo, S. O.; Lemmetyinen, J.; Kein?nen, M.; Keski-Saari, S.; Akkanen, J.; Lepp?nen, M. T.; Wang, Z.; Wang, H.; Price, D. A.; Kukkonen, J. V. K. Response of Lumbriculus variegatus transcriptome and metabolites to model chemical contaminants. Comp. Biochem. Physiol., Part C 157:183-191 (2013) 

    

  74. Zhang, M.; Zhou, Y.; Li T.; Wang, H. L.; Cheng, F.; Zhou, Y. F.; Bi, L. J.*; Zhang, X. E. MutL associates with Escherichia coli RecA and inhibits its ATPase activity. Arch. Biochem. Biophys., 517:98-103 (2012). 

    

  73. Zhang, D.; Shen, H.; Li, G.; Zhao, B. L.; Yu, A.; Zhao, Q.; Wang, H.* Specific and Sensitive fluorescence anisotropy sensing of G-quadruplex structures via a photo-induced electron transfer mechanism. Anal. Chem., 84:8088-8094 (2012). 

    

  72. Han, B.; Zhao, C.; Yin, J.; Wang, H.* High performance aptamer affinity chromatography for single-step selective extraction and screening of basic protein lysozyme. J. Chromatogr. B, 903:112-117 (2012). 

    

  71. Zhang, D.; Zhao, Q.; Zhao, B.; Wang, H.* Fluorescence anisotropy reduction of allosteric aptamer for sensitive and specific protein signaling. Anal. Chem., 84:3070-3074 (2012). 

    

  70. Song, M.; Yuan, S.; Yin, J.; Wang, X.; Meng, Z.; Wang, H.*; Jiang, G. B. Size-dependent toxicity of nano-C60 aggregates: More sensitive indication by apoptosis-related Bax translocation in cultured human cells. Environ. Sci. Technol., 46:3457-3464 (2012). 

    

  69. Zhao, C.; Yin, R. C.; Yin, J. F.; Wang, H.* Capillary monolithic bioreactor of immobilizing snake phosphodiesterase for mass spectrometry based oligodeoxynucleotide sequencing. Anal. Chem., 84:1157-1164 (2012).  

    

  68. Cao, D.; Hu, M.; Han, C.; Yu, J.; Cui, L.; Liu, Y.; Wang, H.; Cai, Y.; Kang, Y.; Zhou, Y. Proton sponge-functionized silica as high performance adsorbents for solid-phase extraction of trace perfluoroalkyl sulfonates in the environmental water samples and their direct analysis by MALDI-TOF-MS. Analyst, 137:2218-2225 (2012). 

    

  67. Zhang, D. P.; Lu, M. L.; Wang, H.* Fluorescence anisotropy analysis for mapping aptamer-protein interactions at the single nucleotide levels. J. Am. Chem. Soc., 133:9188-9191 (2011). 

    

   66. Chen, Z. L.; Lu, M.; Zou, D. D.; Wang, H.* An E. coli SOS-EGFP biosensor for fast and sensitive detection of DNA damaging agents. J. Environ. Sci., 24:541-549 (2012).  

    

  65. Chen, Z. L.; Lu, M.; Zhuang, G. Q.; Wang, H.* Enhanced bacterial biosensor for fast and sensitive detection of oxidatively DNA damaging agents. Anal. Chem., 83:3248-3251 (2011).  

    

  64. Wang X. L.; Suo, Y. S.; Yin, R. C.; Shen, H. Q.*; Wang, H.* Ultra-performance liquid chromatography/tandem mass spectrometry for accurate quantification of global DNA methylation in human sperms. J. Chromatogr. B, 879:1647-1652 (2011). 

    

  63. Yin, J.; Meng, Z.; Zhu, Y.; Song, M.; Wang, H.* Dummy molecularly imprinted polymer for selective screening of bisphenols in river water. Anal. Methods, 3:173-180 (2011).  

    

  62. Yu, Q.; Li, Y.; Ma, A.; Liu, W.; Wang, H.; Zhuang, G. An efficient design strategy for a whole-cell biosensor based on engineered ribosome binding sequences. Anal. Bioanal. Chem., 401:2891-2898 (2011). 

    

  61. Wang, Z.; Wang, X.; Liu, S.; Yin, J.; Wang, H.* Fluorescently imaged particle counting immunoassay for sensitive detection of DNA modifications. Anal. Chem., 82:9901-9908 (2010). (Highlighted in Analytical Chemistry) 

    

  60. Yin, J. F.; Meng, Z. H.; Du, M. J.; Liu, C.; Song, M. Y.; Wang H.* Pseudo-template molecularly imprinted polymer for selective screening of trace ?-lactam antibiotics in river and tap water. J. Chromatogr. A, 1217:5420-5426 (2010). 

    

  59. Lu, M. L.*; Li, X. F.; Le, X. C. Weinfeld, M.; Wang, H.* Identification and characterization of cysteinyl exposure in proteins by selective mercury labeling and nano-electrospray ionization quadrupole time-of-flight mass spectrometry.  Rapid Commun. Mass Spectrom. 24:1523-1532 (2010). 

    

  58. Song, M. Y.; Jiang, G, B.; Yin, J. F.; Wang, H.* Inhibition of polymerase activity by pristine fullerene nanoparticles can be mitigated by abundant proteins. Chem. Comm. 46: 1404-1406 (2010). (Cover) 

    

  57. Lu, M. L.; Mani, R. S.; Karimi-Busheri, F.; Fanta, M.; Wang, H.; Litchfeld, D. W. and Weinfeld, M. Independent mechanisms of stimulation of polynucleotide kinase/phosphatase by phosphorylated and non-phosphorylated XRCC1. Nucleic Acids Res., 38:510-521 (2010).  

    

  56. Li, T.; Zhang, D. P.; Luo, W. R.; Lu, M. L.; Song, Y. L.; Wang, H.* Metal cation mediated capillary electrophoresis of nucleic acids. Anal. Chem., 82:487-490 (2010).  

    

  55. Wang, C.; Li, T.; Wang, Z. X.; Feng, F.; Wang H*. Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N2-dG adducts by capillary electrophoresis immunoassay. J. Chromatogr. A, 1217:2254-2261 (2010). 

    

  54. Wang, H., Lu, M., Tang, M. S., Van Houten, B., Ross, J. B. A., Weinfeld, M.; Le, X.C. DNA wrapping is required for DNA damage recognition in the E. coli DNA nucleotide excision repair pathway. Proc. Natl. Acad. Sci. USA, 106:12849-12854 (2009). 

    

  53. Wang, Z. X.; Lu, M.; Wang, X. L.; Yin, R. C.; Song, Y. L.; Le, X. C.; Wang, H.* Quantum dots enhanced ultrasensitive detection of DNA adducts. Anal. Chem., 81:10285-10289 (2009).  

    

  52. Wang, X. L.; Song, Y. L.; Song, M. Y.; Li, T.; Wang, Z. X.; Wang, H.* Fluorescent polarization combined capillary electrophoresis immunoassay for rapid and sensitive detection of genomic DNA methylation. Anal. Chem., 81:7885-7891 (2009). (Accelerated Article)  

    

  51. Li, T.; Wang, H.* Organic osmolyte mediated capillary electrophoresis for study of protein-DNA interactions. Anal. Chem., 81:1988-1995 (2009). 

    

  50. Gao, H.; Guo, F.; Feng, F.; Yin, J. F.; Song, M. Y.; Wang, H.* Improved preparation and identification of aristolochic acid-DNA adducts by solid-phase extraction with liquid chromatography-tandem mass spectrometry. J. Environ. Sci., 21:1769-1776 (2009). 

    

  49. Lu, M.; Wang, H.; Li, X. F.; Lu, X. F.; Le, X. C. Enzymatic digestion and chromatographic analysis of arsenic species released from proteins. J. Chromatogr. A, 1216:3985-3991 (2009). 

    

  48. Feng, F.; Wang, X. L.; Yuan, H. C.; Wang, H.* Ultra-performance liquid chromatography-tandem mass spectrometry for rapid and highly sensitive analysis of stereoisomers of benzo[a]pyrene diol epoxide-DNA adducts. J. Chromatogr. B, 877: 2104-2112 (2009). 

    

  47. Wang, C.; Feng, F.; Wang, Z. X.; Li, T.; Le, X. C.; Wang, H.* Synthesis and characterization of DNA fluorescent probes containing a single site-specific stereoisomer of anti-benzo(a)pyrene diol epoxide-N2-dG. Chem. Res. Toxicol., 22:676-682 (2009). 

    

  46. Yin, J. F.; Song, Y. L.; Wang, Z. X.; Wang, H.* Fabrication and fluorescence imaging of human low-density lipoprotein coatings for highly efficient capillary electrophoresis separation of basic proteins. Electrophoresis, 30(8):1362-1371 (2009). 

    

  45. Song, M. Y.; Zhang, Y. X.; Li, T.; Wang, Z. X.; Yin, J. F.; Wang, H.* Highly sensitive detection of human thrombin in serum by affinity capillary electrophoresis/laser-induced fluorescence polarization using aptamer as probes. J. Chromatogr. A, 1216:873-878 (2009). 

    

  44. Wang, Z. X.; Wang, C.; Li, T.; Yin, J. F.; Song, M. Y.; Lu, M. L.; Wang, H.* Focusing and stabilization of bis-intercalative dye-DNA complexes for high sensitive capillary electrophoresis-laser induced fluorescence DNA analysis. Electrophoresis, 22:4454-4462 (2008). 

    

  43. Wang, H.*; Wang, Z. X.; Lu, M.; Zou, H. A microdialysis sampling method for evaluation of binding kinetics of small molecules to macromolecules. Anal. Chem., 80:2993-2999 (2008). 

    

  42. Liu, S. F.; Wang, H.*; Song, M. Y.; Yin, J. F.;  Jiang, G. Study of protein binding and micellar partition of highly hydrophobic molecules in a single system using capillary electrophoresis. Electrophoresis, 29:3038-3046 (2008). 

    

  41. Yin, J. F.; Wang, L. J.; Wei, X. Y.; Yang, G. L.; Wang, H. p-tert-butylcalix[8]arene-bonded silica monoliths for liquid chromatography. J. Chromatogr. A, 1188:199-207 (2008).  

    

  40. Lu, M.; Wang, H.; Wang, Z.; Li, X. F.; Le, X. C. Identification of reactive cysteines in a protein using arsenic labeling and collision-activated dissociation tandem mass spectrometry. J. Proteome Res., 7:3080-3090 (2008). 

  . 

  39. Song, M.; Lv, X. F.; Wang, H.; Jiang, G. Fast purification of trace vitellogenin from Chinese rare minnon using protein A immobilized antibody. Anal. Bioanal. Chem. 390:2151-2157 (2008). 

    

  38. Feng, F.; Yin, J. F.; Song, M. Y.; Wang, H.* Preparation, identification and analysis of stereoisomeric anti-benzo[a]pyrene diol Epoxide-deoxyguanosine adducts using phenyl stationary phase based LC-DAD-fluorescence and LC-UV-MS/MS. J. Chromatogr. A, 1183:119-128 (2008). 

    

  37. Yin, J. F.; Yang, G. L.; Wang, H.; Chen, Y. Macroporous polymer monoliths fabricated by using metal-organic coordination gel template. Chem. Commun., 44:4614-4616 (2007). 

    

  36. Feng, F.; Wang, H.* Simultaneous analysis of four stereoisomers of anti-benzo[a]pyrene diol expoxide-deoxyguanosine adducts in short oligodeoxynucleotides using reversed-phase high-performance liquid chromatography.  J. Chromatogr. A, 1162:141-148 (2007). 

    

  35. Lu, M.; Wang, H.; Li, X. F.; Arnold, L.L.; Cohen, S.M.; Le, X.C. Binding of dimethylarsinous acid to cysteine 13 in the chain of rat hemoglobin is responsible for retention of arsenic in rat blood. Chem. Res. Toxicol., 20:27-37 (2007). (One of the 5 most cited articles in this journal in 2007) 

                 

  34. Shen, S. W.; Lee, J.; Sun, X.; Wang, H.; Weinfeld, M.; Le, X. C. Elevation of cellular BPDE uptake by human cells: a possible factor contributing to co-carcinogenicity by arsenite. Environ. Health Perspect., 114:832-1837 (2006).   

    

  33. Xiao, H.; Li, X., Zou, H.; Yang, L.; Yang, Y. Q.; Wang, Y. L.; Wang, H.; Le XC. CE-LIF coupled with flow cytometry for high-throughput quantitation of fluorophores in single intact cells. Electrophoresis 27:3452-3459 (2006). 

    

  32. Xiao, H.; Li, X., Zou, H.; Yang, L.; Yang, Y. Q.; Wang, Y. L.; Wang, H.; Le XC. Immunoassay of P-glycoprotein on single cell by capillary electrophoresis with laser induced fluorescence detection. Anal. Chim. Acta, 556:340-346 (2006). 

    

  31. Wang, H.; Lu, M.; Le, X.C. DNA-driven focusing for protein-DNA binding assays using capillary electrophoresis. Anal. Chem., 77:4985-4990 (2005). 

    

  30. Le, X. C.; Pavski, V.; Wang, H. Affinity recognition, capillary electrophoresis and laser induced fluorescence polarization for ultrasensitive bioanalysis. Can. J. Chem., 83:185-194 (2005). 

                 

  29. Lu, M.; Wang, H.; Li, X. F.; Lu X.; Le, X.C. Evidence of hemoglobin binding to arsenic as a basis for the accumulation of arsenic in rat blood. Chem. Res. Toxicol., 17:1733-1742 (2004). 

    

  28. Wang, H.; Lu, M.; Mei, N.; Lee, J.; Weinfeld, M.; Le, X.C. Immunoassay using Capillary electrophoresis laser induced fluorescence detection for DNA adducts. Anal. Chim. Acta, 500:13-20 (2004). 

    

  27. Wang, H.; Lu, M.; Weinfeld, M.; Le, X.C. Enhancement of immunocomplex detection and application to assays for DNA adduct of benzo(a)yrene. Anal. Chem., 75:247-254 (2003). 

    

  26. Wang, H.; Xing, J.; Tan, W.; Lam, M.; Carnelley, T.; Weinfeld, M.; Le, X.C. Binding stoichiometry of DNA adducts with antibody studied by capillary electrophoresis and laser-induced fluorescence. Anal. Chem., 74: 3714-3719 (2002). 

    

  25. Carnelley, T.J.; Barker, S.; Wang, H.; Tan, W.G.; Weinfeld, M.; Le, X.C. Synthesis, characterization, and applications of a fluorescent DNA damage probe. Chem. Res. Toxicol., 14: 1513-1522 (2001). 

    

  24. Tan, W.G.; Carnelley, T.J.; Murphy, P.; Wang, H.; Lee, J.; Barker, S.; Weinfeld, M.; Le, X.C. Detection of DNA adducts of benzo[a]pyrene using immuno-electrophoresis with laser-induced fluorescence: analysis of A549 cells. J. Chromatogr. A, 924:377-386 (2001).  

    

  23. Guo, M.; Zou H.; Wang, H; et. al.  Binding of metal ions with protein studied by a combined technique of microdialysis with liquid chromatography. Anal. Chim. Acta, 443:91-99 (2001). 

    

  22. Kong, L.; Li, X.; Zou, H.; Wang H.; Mao, X.-Q.; Zhang, Q.; Ni, J.Y.  Analysis of terpene compounds in Cimicifuga foetida L. by reversed-phase high-performance liquid chromatography with evaporative light scattering detection. J. Chromatogr. A, 936:111-118 (2001). 

    

  21. Zhang, Q.; Zou, H.; Chen, X.; Wang, H.; Luo, Q.; Ni, J.  Synthesis and Characteristics of the human serum albumin-triazine chiral stationary phase.  Chirality, 12:714-719 (2000). 

    

  20. Wang, H.; Zou, H.; Ni, J.; Guo, B.  Comparative separation of biological active components in Rhizoma ChuangXiong on human serum albumin and ?1- acid glycoprotein stationary phases in molecular chromatography. Chromatographia,  52:459-464 (2000). 

    

  19. Zhou, D.; Zou, H.; Wang, H.; Zhang Q.; Ni, J.  Alkaline treatment of the cellulose fiber affecting membrane column behavior for high-performance immunoaffinity chromatography. Biomed. Chromatogr., 14:511-515 (2000). 

    

  18. Jiang, H.; Zou, H.; Wang, H.; Zhang, Q.; Ni, J.; Zhang, Q.-C.; Guo, Z.; Chen, X. Combination of MALDI-TOF mass spectrometry with immobilized enzyme microreactor for peptide mapping. Science in China (Series B), 43:625-633 (2000). 

    

  17. Chen, X.; Zou, H.; Yang, L.; Wang, H.; Zhang, Q. Chiral high performance liquid chromatography with cellulose trisphenylcarbamate coated on underivatised silica.  Am. Lab., 32:30 (2000). 

    

  16. Jiang, H.; Zou, H.; Wang, H.; Ni J.; Zhang, Y. On-line characterization of the activity and reaction kinetics of immobilized enzyme by high performance frontal analysis. J. Chromatogr. A, 903:77-84 (2000). 

    

  15. Chen, X.; Zou, H.; Yang, L.; Wang, H.; Zhang Q. Optical separation of ?-alkyl phenyl acetonitriles by HPLC on triacetate cellulose chiral stationary coated on underivatized silica, Chirality, 12: 621-626 (2000). 

    

  14. Wang, H.; Zou, H.; Kong, L.; Gao, S.; Guo, B. Fractation and analysis of Artemisia capillaris Thunb. by molecular biochromatography with human serum albumin stationary phase.  J. Chromatogr. A, 870:501-590 (2000). 

    

  13. Zhang, Q.; Zou, H.; Wang, H.; Ni, J.  Synthesis of a silica-bonded BSA s-triazine chiral stationary for high-performance liquid chromatographic resolution of enantiomers.  J. Chromatogr. A, 866:173-181 (2000). 

    

  12. Wang, H.; Kong, L.; Zou, H.; Ni, J.; Zhang, Y. Screening and analysis of biologically active compounds in Angelica Sinensis by molecular biochromatography.   Chromatographia, 50:439-445 (1999). 

    

  11. Wang, H.; Zou, H.; Zhang, Y.; Pang H.; Su, C. Y.; Liu, G,; Hui, M.; Fu, L. Determination of Ginsenoside Rg3 in plasma by solid phase extraction and high performance liquid chromatography for pharmacokinetic study.   J. Chromatogr. B, 731:403-409 (1999). 

    

  10. Zhou, D.; Zou, H.; Ni, J.; Wang, H.; Yang L.; Zhang Y. Membrane affinity chromatography for analysis and purification of biopolymers. Chromatographia, 50:27-34 (1999). 

    

  9. Zou, H.; Wang, H.;  Zhang, Y.  Drug-protein interaction studied by technique of microdialysis combined with high performance liquid chromatography. Reviews in Anal. Chem., 18:383-407 (1999). 

    

  8. Wang, H.; Zou, H.; Zhang, Y. Quantitative study of competitive binding of drugs to protein by microdialysis/high-performance liquid chromatography. Anal. Chem., 70:373-377 (1998).  

    

  7. Wang, H.; Zou, H.; Zhang, Y. Multi-Site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography. Biomed. Chromatogr., 12:4-7 (1998). 

    

  6. Zou, H.; Wang, H.; Zhang, Y.  Stereoselective binding of warfarin and ketoprofen to human serum albumin determined by microdialysis combined with HPLC. J. Liq. Chromatogr. &  Rel. Technol., 21:2663-2674 (1998). 

    

  5. Wang, H.; Zou, H.; Zhang, Y. Determination of drug- protein interaction studied by combined microdialysis and high performance liquid chromatography.  Chromatographia, 44:205-208 (1997). 

    

  4. Wang, H.; Zou, H.; Feng; A.; Zhang; Y. Binding of sulfamethoxazole to human serum albumin by a combined technique of microdialysis with high performance liquid chromatography. Anal. Chim. Acta, 342:159-165 (1997). 

    

  3. Wang, H.; Zou, H.; Zhang Y. Study of competitive interaction between drugs and human serum albumin by Microdialysis/HPLC.  Science in China (Series B), 40:643-649 (1997).  

    

  2. Wang, H.; Li, T.; Zou, H.; Zhang, Y.; Chao, J.; Chao, L. The purification of human urinary kallikrein with ion-exchange radial flow membrane chromatography. Biomed. Chromatogr., 10:139-143 (1996). 

    

  1. Zou, H.; Wang, H.; Lee, R.; Zhang, Y.  Separation mechnism of micellar-electrokinetic capillary chromatography studied by quantitative-structure retentionship. Progress in Nature Science, 6: 683-691 (1996). 


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